PULLULANASE (Crystal)

Pullulan 6-Glucanohydrolase

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  • Code No.
    EN202
  • Enzyme Commission numbers
    EC 3. 2. 1. 41
  • Origin
    Klebsiella pneumoniae
  • Characteristic
    Hydrolyzes the α-1,6-glucosidic linkages in amylopectin and pullulan
  • Enzyme Unit
    One unit liberates 1.0 μmole of maltotriose from pullulan at pH 5.0 and 30℃ per minute
  • Optimum pH
    5.0 ～　5.5
  • Use
    Identification of pullulan
  • Purity
    Electrophoretically pure
  • Specific Activity
    Not less than 30 units/mg protein
  • α-amylase activity
    Not less than 0.5
  • pH
    5.5～6.5
  • Appearance
    Crystalline suspension in 3.2M ammonium sulfate solution
  • Storage
    Store at 0 ～　4℃
  • Availability
    1 mg (1 ml)
  • References
    Arch. Biochem. Biophys.,137, 483～493 (1970)
    Biochim. Biophys. Acta,268, 497～505 (1972)
    Biochim. Biophys. Acta,293, 197～202 (1973)
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