Reagent for cell biology Cell proliferation assay

Cell Proliferation Assay Reagent

Resazurin can be used quantitatively determine cell proliferation, viability, and cytotoxicity.
Resazurin, when added to viable cells, is reduced by the cellular enzymatic or chemical reactions converting blue/non-fluorescent resazurin to highly fluorescent resorufin.
The assay is simple to perform since the indicator is water-soluble and has low toxicity, thus eliminating the washing/fixing and extraction steps required in other commonly used cell proliferation assays.

Product

R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]

Application

1. Add R0195 at a volume equal to 10% of the cell culture media volume.
2. Return cells to the incubator and continue the incubation for 2-24 hours*.
3. Measure the fluorescent intensity using 540-570 nm excitation and 590 nm emission wavelengths.
＊Absorbance can be measured using a spectrophotometer set at 570 nm.
Resazurin may be added at any time point during the culture period. For measurement of cell proliferation, it is best to add resazurin during the log phase of growth.