Reagent for cell biology, Fluorescent stain

Applications

(A) The HeLa cells were incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG) and were further incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] and Streptavidin FITC Conjugate [S0966] ( green fluorescence ). And then the nuclei was stained with DAPI·2HCl [A2412] ( blue fluorescence ) .
(Laser Scanning Microscope: Olympus FLUOVIEW FV 3000)




(B) The nuclei of HeLa cells was stained with Bisbenzimide H 33258 [H1343] ( blue fluorescence ). α-Tubulin was stained with anti-α-tubulin antibody and Goat Anti-Mouse IgG Biotin Conjugate [G0387] and Streptavidin R-PE Conjugate [T3885] ( red fluorescence ). Mitochondria was stained with primary antibody and Goat Anti-Rabbit IgG FITC Conjugate [G0452] ( green fluorescence )**
(Laser Scanning Microscope: Olympus FLUOVIEW FV 3000)



(C) The HeLa cells were incubated with Mouse Anti-CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line). Subsequently, both were stained with Goat Anti-Mouse IgG Biotin Conjugate [G0387] and Streptavidin R-PE Conjugate [T3885].
(Flow cytometer: Sysmex RF-500)

**Please refer to the product page for staining procedure.
R-PE/FITC-labeled anti-Mouse IgG or anti-Rabbit IgG antibodies and streptavidins can be used for fluorescence immunostaining and flow cytometry.