[{"data":1,"prerenderedAt":232},["ShallowReactive",2],{"6eGX28z45i":3,"X1VVWOlEHW":105},[4],{"id":5,"date":6,"date_gmt":7,"guid":8,"modified":10,"modified_gmt":11,"slug":12,"status":13,"type":14,"link":15,"title":16,"content":18,"featured_media":21,"menu_order":22,"template":23,"lifescience_cat":24,"class_list":26,"acf":33,"lang":64,"translations":65,"pll_sync_post":68,"_links":69},902,"2021-06-26T00:10:25","2021-06-25T15:10:25",{"rendered":9},"https://nagase-labobio-mpd.gear5.jp/?post_type=lifescience&p=902","2024-04-10T01:16:27","2024-04-09T16:16:27","cubic-hv","publish","lifescience","https://nagase-labobio-mpd.gear5.jp/th/lifescience/cubic-hv/",{"rendered":17},"CUBIC-HV",{"rendered":19,"protected":20},"\u003Cp>ชุดอุปกรณ์สารทดลองย้อมสีเนื้อเยื่อแบบ 3 มิติ\u003Cbr />\nCUBIC-HV เป็นเทคโนโลยีใหม่ซึ่งเป็นการพัฒนาครั้งสำคัญที่ทำให้สามารถย้อมสีเนื้อเยื่อแบบ 3 มิติและเฝ้าสังเกตได้ในอวัยวะภายในทั้งหมดทั่วร่างกาย (ซึ่งพัฒนาโดย ศ.อูเอดะ และ รศ.ชูซากิแห่งสถาบันวิจัย RIKEN มหาวิทยาลัยโตเกียว) โปรโตคอลนี้เป็นการนำเสนอวิธีการย้อมสีเนื้อเยื่อแบบ 3 มิติที่มีประสิทธิภาพสูง บริษัท CUBICStars จำกัด มีใบอนุญาตทางด้าน CUBIC-HV และได้ผลิตชุดอุปกรณ์สารทดลองดังกล่าวอยู่ โดยได้จัดเตรียมผลิตภัณฑ์ไว้ 2 รายการตามวิธีการนำไปใช้\u003Cbr />\n\u003C/p>\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero caption\">＜Note＞\u003Cbr />\nDifferences between CUBIC-HV™1 3D Immunostaining kit (product code: C3708) and CUBIC-HV™1 3D immunostaining kit (casein separately) (product code: C3717) ;\u003Cbr />\nThe CUBIC-HV™1 3D Immunostaining kit (product code: C3708) contains casein dissolved in immunostaining buffer and can be used immediately. The immunostaining buffer should be used as soon as possible.\u003Cbr />\nCUBIC-HV™1 3D immunostaining kit (Casein separately) (product code: C3717) does not contain casein dissolved in the immunostaining buffer and is enclosed in a separate package. Mix casein into the immunostaining buffer immediately before use, and use the buffer as soon as possible after adding casein. It takes about 2 hours at room temperature to dissolve the casein.\u003C/p>\n",false,2938,0,"",[25],43,[27,14,28,29,30,31,32],"post-902","type-lifescience","status-publish","has-post-thumbnail","hentry","lifescience_cat-saibou_th",{"product_archive":34,"product_number":23,"sds_link":39,"product_table":40,"product_info_ttl":37,"product_info_txt":62,"product_info_img":23,"product_references":63},[35],{"product_type":36,"product_cat":37,"product_overview":38,"product_name":17},"สารทดลองทางชีววิทยาของเซลล์","ชุดอุปกรณ์สารทดลองย้อมสีเนื้อเยื่อแบบ 3 มิติ","ทำให้สามารถย้อมสีเนื้อเยื่อแบบ 3 มิติและเฝ้าสังเกตได้ในอวัยวะภายในทั้งหมดทั่วร่างกาย","https://www.tcichemicals.com/TH/en/product/topics/CUBIC-HV",[41,51,57],{"product_table_code":42,"product_table_name":43,"product_table_capacity":44,"sds_table_check":45,"sds_table_link":47,"protocol_table_check":48,"protocol_table_link":50},"C3709","CUBIC-HV™1 3D nuclear staining kit","1 kit",[46],"SDS","https://nagase-labobio-mpd.gear5.jp/wp/wp-content/uploads/2021/06/C3709_SDS.pdf",[49],"Protocol","https://nagase-labobio-mpd.gear5.jp/wp/wp-content/uploads/2021/06/CUBIC_CUBIC-HV_Protocol-en.pdf",{"product_table_code":52,"product_table_name":53,"product_table_capacity":44,"sds_table_check":54,"sds_table_link":55,"protocol_table_check":56,"protocol_table_link":50},"C3708","CUBIC-HV™1 3D immunostaining kit",[46],"https://nagase-labobio-mpd.gear5.jp/wp/wp-content/uploads/2021/06/C3708_SDS.pdf",[49],{"product_table_code":58,"product_table_name":59,"product_table_capacity":44,"sds_table_check":60,"sds_table_link":55,"protocol_table_check":61,"protocol_table_link":50},"C3717","CUBIC-HV™1 3D immunostaining kit (Casein separately)",[46],[49],"\u003Cp class=\"product-post-detail-info-tech-title\">CUBIC-HV™1 3D Immunostaining kit (product code: C3708)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Process outline\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Delipidation\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Perfusion fixation and dissection of the mouse brain\r\nPost-fix \u003C24 h\r\nWash (PBS) 2 h x3\r\nDelipidation (CUBIC-L; Product No. T3740) 3-7 days\r\nWash (PBS) 2 h x3\u003C/p>\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days\r\nWash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3\r\n\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Optional process; Enzyme reaction\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Primary antibody (anti-NeuN antibody in C3708 kit can be used): 5 µg/whole brain\r\nSecondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain\r\nPrimary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h\r\nImmunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week\r\nReaction in the same buffer at 4°C, 1 day\r\nWash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3\u003C/p>\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Fix and RI matching\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Post-fix (in 1% Formaldehyde Solution), 1 day\r\nWash (PBS) 2 h\r\nRI matching (CUBIC-R+; Product No. T3740), \u003C3 days\r\nMicroscopic observation\u003C/p>\r\n\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Related Products\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero fwb\">T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]\r\nT3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]\r\nC3708 CUBIC-HV™1 3D immunostaining kit\r\nF0622 37% Formaldehyde Solution\r\nH0396 HEPES\u003C/p>\r\n\r\n\r\n\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-title\">CUBIC-HV™1 3D nuclear staining kit (Product No. C3709)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Process outline\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Delipidation\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Perfusion fixation and dissection of the mouse brain\r\nPost-fix \u003C24 h\r\nWash (PBS) 2 h x3\r\nDelipidation (CUBIC-L; Product No. T3740) 3-7 days\r\nWash (PBS) 2 h x3\u003C/p>\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days\r\nWash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3\u003C/p>\r\n\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Optional process; Enzyme reaction\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708)\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Primary antibody (anti-NeuN antibody in C3708 kit can be used): 5 µg/whole brain\r\nSecondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain\r\nPrimary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h\r\nImmunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week\r\nReaction in the same buffer at 4°C, 1 day\r\nWash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3\u003C/p>\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-underline\">Fix and RI matching\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero\">Post-fix (in 1% Formaldehyde Solution), 1 day\r\nWash (PBS) 2 h\r\nRI matching (CUBIC-R+; Product No. T3740), \u003C3 days\r\nMicroscopic observation\u003C/p>\r\n\r\n\r\n\u003Cp class=\"product-post-detail-info-tech-subtitle\">Related Products\u003C/p>\r\n\u003Cp class=\"product-post-detail-info-content-txt pt-zero fwb\">T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]\r\nT3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]\r\nC3708 CUBIC-HV™1 3D immunostaining kit\r\nF0622 37% Formaldehyde Solution\r\nH0396 HEPES\u003C/p>","Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues\r\n\u003Ca href=\"https://doi.org/10.1038/s41467-020-15906-5\" target=\"_blank\" rel=”noopener noreferrer”>E. A. Susaki, C. Shimizu, A. Kuno, K. Tainaka, X. Li, K. Nishi, K. Morishima, H. Ono, K. L. Ode, Y. Saeki, K. Miyamichi, K. Isa, C. Yokoyama, H. Kitaura, M. Ikemura, T. Ushiku, Y. Shimizu, T. Saito, T. C. Saido, M. Fukayama, H. Onoe, K. Touhara, T. Isa, A. Kakita, M. Shibayama, H. R. Ueda, Nat. Commun. 2020, 11, 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