Cholesterol
Summary of the Test Item
• Levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) are test markers for disorders such as dyslipidemia.
• Cholesterol levels are assayed using the enzymatic method.
Nagase Diagnostics Solutions
• We supply cholesterol esterase and cholesterol oxidase for the cholesterol levels measurement. Our products include two types of cholesterol esterase (CENⅡ and CEBPⅡ) with different properties, which may be used as appropriate depending on the target of the assay (TC, HDL-C, or LDL-C) and/or the formulation.
• With a highly hydrophobic enzyme such as cholesterol esterase, it is important to select appropriate addtives.
We have data on the surfactants and salts that increase the activities and stabilities of CENⅡ and CEBPⅡ, which may facilitate the acceleration of your product development.
Please contact us for details.
Measurement Principle
Performance Comparison Chart of Nagase Diagnostics Enzymes
| PRODUCTS | Cholesterol Esterase | Cholesterol Oxidase | |
|---|---|---|---|
CENⅡ | CEBPⅡ | CONⅡ-FD (T-101) | |
Characteristics | High activity, | Low adsorption | Low Km value |
Optimum pH | 6.5 | 5.6 | 7.0-7.5 |
pH stability | pH stability | Stable in low pH | 5.7-7.8 |
Thermal stability | Stable at <45℃ | Stable at <45℃ | Stable at <65℃ |
Related Information
CENⅡRelated Information
・Result of NEDO Smart Cell Project leads to commercial product (asahi-kasei.com)
・Awarded the 71st Okochi Memorial Technology Prize (Nagase Diagnostics LinkedIn)
Product Information
Please contact us if you are looking for other products not listed below.
Nagase Diagnostics carries many solutions and products.
We may be able to meet your needs.